culture cell meaning in English
培养细胞
Examples
- Upon exposure the culture cells to 5 mm sodium butyrate , the growth of lepcs was significantly arrested
Dmso和丁酸钠是两种最常用的小分子细胞分化诱导剂。 - 1 . 1 observation of culture cells with phase contrast microscope the smg cells cultured are polygon epithelium cells and could go down to 3 to 4generations and live up to 3 - 4w in the medium ( dme
1 .相差倒置显微镜下观察smg细胞生长期间呈扁平状，单层生长，为多角形的上皮细胞，细胞可传代生长3一4代，存活3一4w 。 - The results showed that : when cultured in the medium of m199 , supplemented with 20 % bovine serum containing a moderate amount of antibiotics , the incubtion ph 7 . 2 - 7 . 4 , the culture temperature 28 . the primary culture cells formed the dense single wall within three weeks , the generation time of the subculture cells was 5 - 6 days , most cultured cells were fibroblastic - like cells with few epithelial - like cells
研究初步表明：在m199培养基加入20小牛血清(常规量双抗) ， ph在7 . 2 - 7 . 4之间， 28的培养条件，四倍体鲫鲤鱼肾组织原代培养三周左右即可形成致密单层，传代细胞为5 - 6天左右传一次。培养细胞以成纤维样细胞为主，有少数上皮样细胞。 - The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna , which was first linearized by aocl . after two rounds of plaque isolation , the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization . the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10
重组转移载体dna与经bsu36i酶切线性化的修饰型病毒bm - bacpakdna共转染家蚕bmn细胞，两轮空斑筛选和纯化，获得重组病毒rbmbacph - egf ，经pcr扩增、 dna点杂交等方法鉴定证实重组病毒中已正确插入ph - egf融合基因。 - Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently , setting up to express system efficiently , suspending culture cell , can raise the cell density in the scale turn the production , strengthen the cell vitality , control cell to propagate level , extension cell culture period , increase the target protein of yield , raise product quality , simplification of produces technics , reduce production cost , then raising the efficiency that the scale turns culture
提要：采用无血清无动物组分培养基驯化高效表达细胞，构建高效表达系统，悬浮培养细胞，可以在规模化生产中，提高细胞密度，增强细胞活力，控制细胞增殖水平，延长细胞培养周期，增加目标蛋白的产量，提高产品质量，简化生产工艺，降低生产成本，进而提高规模化培养的效能。